MicrobialTrack
MALDI-TOF MS 微生物鑑定軟體
MALDI-TOF MS 微生物鑑定平台

MicrobialTrack 是一款全新的分析平台,運用由基因組序列預測的龐大原核生物蛋白質理論質量資料庫,結合 MALDI-TOF MS (基質輔助雷射游離飛行時間質譜)技術,進行原核微生物的鑑定。該資料庫涵蓋了大量先前無法鑑定的細菌與古菌的系統分類群,使得無法培養或難以培養的微生物也能被鑑定。由於 MicrobialTrack 所採用的鑑定演算法依賴於基因組預測蛋白質的質量,因此其鑑定效能不會受到培養條件或 MALDI-TOF MS 儀器型號的主要影響。此外,MicrobialTrack 不僅提供精確的微生物鑑定結果,亦可透過理論質量進行深入的質譜數據分析,進一步為偵測到的質譜訊號賦予對應的蛋白質名稱與相關資訊。
eMSTAT Solution 為島津製作所及其關係企業在日本及其他國家的註冊商標。
SampleStation 與 AuraSolution 為 Kratos Analytical Ltd. 的註冊商標。
特色
影片
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MicrobialTrack MALDI-TOF MS Microbial Identification Software Interview with Yuji Sekiguchi Ph.D.
MicrobialTrack is a new analytical platform for MALDI-TOF MS-based identification of prokaryotic microorganisms. A vast database of theoretical prokaryotic protein masses predicted from genome sequences and an original identification algorithm enable the rapid identification of a wide range of microbial species, independent of culture conditions or MALDI-TOF MS models. In addition to identification results, MicrobialTrack conducts a detailed mass spectral analysis using theoretical protein masses to assign proteomic information.
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Sample prep for MicrobialTrack
This video describes the sample preparation procedures for microbial identification using MALDI-TOF MS and MicrobialTrack.
It covers all major steps, including preparation of reagents and equipment, matrix solution preparation, and preparation of test and calibration samples. Important points and notes for each step are also provided.